Services

Electron Microscopy

Sample Preparation

TEM

• Post Fixation
• Plastic Embedding
• Ultramicrotome sectioning and staining

SEM

• Silver painting
• Sputter Coating

Imaging

Staff can assist users to acquire their own images.  Visit the Electron Microscopy page for details about each machine.  See Pricing page for rates.

Training

Both SEM and TEM microscopes are available for independent usage once training is completed.  Contact Sarah Abrahamson for training details and availabilities (sarah.abrahamson@und.edu; 701-777-3399) .

Samples for training will be provided by the Imaging Core for training purposes only.  Should the user wish to train with their own samples, they will be billed the hourly usage rate for the equipment they are utilizing in accordance with the current Imaging Core’s pricing schedule.

Light Microscopy

Imaging

We offer a wide variety of light microscopy imaging services including both brightfield and fluorescent imaging.  Visit the Light Microscopy page for details about each machine.  See Pricing page for rates.

• Note: The Leica Stellaris Confocal, Leica DMi8 Thunder Imager, and Olympus IX83 TIRF microscopes are all Zoom compatible.

Training

Independent usage is available for any of the light microscopes after training has been completed.  Contact Sarah Abrahamson for training details and availabilities (sarah.abrahamson@und.edu; 701-777-3399).

Samples for training will be provided by the Imaging Core for training purposes only.  Should the user wish to train with their own samples, they will be billed the hourly usage rate for the equipment they are utilizing in accordance with the current Imaging Core’s pricing schedule.

Requesting Service Procedure

We highly recommend you contact us prior to filling out the form if you have questions.

• • Light Microscopy: Sarah Abrahamson (sarah.abrahamson@und.edu; 701-777-3399)

To request any of the Imaging Core's Services please do the following:

1. 1. Complete the online Sample Submission Form found below.
   2. Imaging Core staff will contact you for remaining details on your experiment as well as a project request number.
   3. Prepare your samples and clearly print the provided project request number on a label on the outside of the container.
      
      • All samples must be submitted inside of an appropriate BSL level container.
   4. Drop off your samples on the cart outside of the Imaging Core (SMHS suite W162) at the agreed upon drop off time.
      
      • Fixed samples on slides must be completely cured, dry (if sealed), and clean.  Samples that do not comply must be picked up, corrected, and placed back on the cart outside of the Imaging Core before services can begin.
   5. Imaging Core staff will complete your service and contact you to coordinate a pick up time.  If you requested imaging services, they will be made available on the Imaging Core server.
   6. Pick up your sample from the cart outside of the Imaging Core.

Forms and Links

• Sample Submission Form
• Coverslip Sealing Example Guide
• Invitrogen Fluorescence Spectra Viewer

Acknowledgment

Facility users must acknowledge the Imaging Core facility in any work that reports data collected and processed using either core equipment or staff assistance.  The facility is supported by federal funds, which must be acknowledged.

Based on NIH attribution guidelines, users are required to use the following attribution statement:

“Imaging studies were conducted in the UND Imaging Core facility supported by NIH grant P20GM113123, DaCCoTA CTR NIH grant U54GM128729, and UNDSMHS funds."

Please note also that NIH requires that all articles arising from NIH supported work (including work in NIH supported core facilities) must be deposited in PubMed Central and have a PMCID. Please adhere to this policy.