Equipment Available
Quality Control
Agilent 4200 Tapestation – The Agilent TapeStation is an automated electrophoresis for quality assessment of DNA and RNA through analysis of fragment size distribution.
Thermo Scientific NanoDrop One – NanoDrop One is a UV-visible spectrophotometer suitable for measuring sample purity of RNA and DNA.
Invitrogen Qubit 4 Fluorometer – The Qubit 4 quantitates DNA and RNA with unprecedented accuracy and sensitivity.
Nexcelom Cellometer – The Nexcelom Cellometer is an automated cell counter capable of autofocusing and providing accurate cell counts and viability analysis.
Sequencing Platforms
Illumina MiSeq – The MiSeq is a short-read sequencer for focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, and amplicon sequencing. MiSeq sequencing kits enable up to 15 Gb of output with 25 M sequencing reads and 2x300 bp read lengths.
GridION X5 Nanopore – The GridION is long-read sequencer for applications such as direct sequencing of DNA and RNA, identification of modified bases (mC and hmC), long-range genomic variations including phase variants, the genomic context of copy number variants (CNVs), exact breakpoints of inversions, insertions, deletions and translocations, 16S metagenomics, and long reads for small genome assembly. The GridION is capable of generating 150 Gb of data per run.
GEOMx Digital Spatial Profiler – The GEOMx allows the user to image and profile gene expression and protein levels on any slide-mounted tissue, FFPE or fresh frozen. Use any morphology marker, with pre-defined data processing pipelines, interactive data analysis and accessible biological insights.
10X Chromium iX Single Cell Controller – The 10X efficiently combines large cell numbers with a massively diverse barcode library to generate >100,000 barcode-containing cells in a matter of minutes. These barcoded cells are compatible with Chromium Solution, from genome to single cell analysis and useful for single-cell expression profiling, ATAC-Seq, exome sequencing, CNV detection single cell, de novo genome assembly.
RNA/DNA Extraction
Promega Maxwell RSC Automated Extractor - The Maxwell RSC is a compact, automated instrument that can process up to 16 samples simultaneously for nucleic acid purification. It offers consistent and reliable RNA or DNA extraction.
Instructions for equipment use and Rules
Equipment is available on a first-come, first-serve basis and the usage time must be reserved using your Infinity account. If you do not have an Infinity account, please contact Core staff. To request work on samples with an instrument only available to the Core staff, please refer to the “Sample Submission” tab in the side menu for instructions.
*Some instruments will require a training session before unsupervised use is allowed. These will be indicated as such when creating a reservation within Infinity.
*The core reserves the right to remove any user who is found utilizing equipment without permission or damaging equipment and will remove any permissions for future use from said user.
Contact: Core staff