Services

The Mass Spectrometry lab is mainly focused on small molecule research (up to 4,000 Da).

Services

Developing sample preparation protocols
Training users for sample preparation
Developing LC-MS, GC-MS, or direct infusion-MS methods
Performing M.S. analyses for the users
Developing the quantification method, and also developing alternative approaches for data analysis and interpretation (e.g.: metabolite ID, structure determination, metabolomic profiling)
Training users to use the quantification or other developed methods for data analysis

Example Analyses

Quantitative Analysis of Small Molecules by UPLC or HPLC Coupled with Tandem Mass Spectrometry
- Bioactive lipids
  - Prostaglandin profiling (COX products)
  - Products of lipid peroxidation (isoprostanes)
  - Hydroxy-Fatty Acids profiling including resolvins (LOX and P450 products)
- Hydroxycholesterol quantification (Link 1, Link 2)
- Carbohydrates
- Nucleotides and nucleosides (adenosine, DNA modifications)
- Lipid profiling
- HNE (products of fatty acid peroxidation)
Qualitative Analysis of Small Molecules by Direct Infusion or UPLC Analysis Coupled with Tandem Mass Spectrometry
- Structure and elemental composition prediction using high resolution accurate mass analysis
Metabolomics (comparison of metabolites between different experimental groups e.g. wild-type and genetically modified, control and treated)
Drug Metabolism (determination of molecule biotransformations)
Qualitative and Quantitative Analysis of Small Volatile Molecules Using GC/MS with Various Ionizations techniques, e.g., EI, NICI, CI.

Operation

We are using two models of operation:

Full Service: The Core Facility provides full service for the above analyses with the exception of LC method development, sample extraction, sample preparation, and data analysis that may be available through collaboration.
Self Service: Work-in users are allowed. The training of work-in users is provided by the Core Facility Personnel. Training is required to ensure protection of instruments and optimization of results. The training is scheduled on an individual basis.

Fees

Service Fees for Mass Spec Facility	Rate Per Sample/Service: NDUS Users	Rate Per Sample/Service: Non-NDUS Users
Small molecules 1. Simple sample analysis (up to 4 analytes) G2S/TQS/PolarisQ	$12	$30
2. Complex sample analysis (5 or more analytes) G2S/TQS/PolarisQ	$16	$45
3. Method development, simple sample analysis (up to 4 analytes) with UND maintaining IP rights G2S/TQS PolarisQ	$300 $200	$500 $300
4. Method development, complex sample analysis (5 or more analytes) with UND maintaining IP rights G2S/TQS PolarisQ	$900 $550	$1,500 $1,000
Protein analysis 1. Simple sample (up to 5 proteins) G2S	$30	$75
2. Complex sample (5 or more proteins) G2S	$45	$100
3. Method development with UND maintaining IP rights G2S	$600	$1,500

Service Fees for Mass Spec Facility

Rate Per Sample/Service:
NDUS Users

Rate Per Sample/Service:
Non-NDUS Users

Small molecules

1. Simple sample analysis (up to 4 analytes)

G2S/TQS/PolarisQ

$12

$30

2. Complex sample analysis (5 or more analytes)

G2S/TQS/PolarisQ

$16

$45

3. Method development, simple sample analysis (up to 4 analytes) with UND maintaining IP rights

G2S/TQS
PolarisQ

$300
$200

$500
$300

4. Method development, complex sample analysis (5 or more analytes) with UND maintaining IP rights

G2S/TQS
PolarisQ

$900
$550

$1,500
$1,000

Protein analysis

1. Simple sample (up to 5 proteins)

$30

$75

2. Complex sample (5 or more proteins)

$45

$100

3. Method development with UND maintaining IP rights

$600

$1,500

Volume Discounts

Volume discounts are available on a case-by-case basis.

Financial Support

To apply for support funds, faculty may download the fee support application and send the completed form via e-mail to Dr. Mikhail Golovko at Mikhail.Golovko@UND.edu

Mass Spectrometry Support Application