Services
The Mass Spectrometry lab is mainly focused on small molecule research (up to 4,000 Da).
Services
- Developing sample preparation protocols
- Training users for sample preparation
- Developing LC-MS, GC-MS, or direct infusion-MS methods
- Performing M.S. analyses for the users
- Developing the quantification method, and also developing alternative approaches for data analysis and interpretation (e.g.: metabolite ID, structure determination, metabolomic profiling)
- Training users to use the quantification or other developed methods for data analysis
Example Analyses
- Quantitative Analysis of Small Molecules by UPLC or HPLC Coupled with Tandem Mass
Spectrometry
- Bioactive lipids
- Prostaglandin profiling (COX products)
- Products of lipid peroxidation (isoprostanes)
- Hydroxy-Fatty Acids profiling including resolvins (LOX and P450 products)
- Hydroxycholesterol quantification (Link 1, Link 2)
- Carbohydrates
- Nucleotides and nucleosides (adenosine, DNA modifications)
- Lipid profiling
- HNE (products of fatty acid peroxidation)
- Bioactive lipids
- Qualitative Analysis of Small Molecules by Direct Infusion or UPLC Analysis Coupled
with Tandem Mass Spectrometry
- Structure and elemental composition prediction using high resolution accurate mass analysis
- Metabolomics (comparison of metabolites between different experimental groups e.g. wild-type and genetically modified, control and treated)
- Drug Metabolism (determination of molecule biotransformations)
- Qualitative and Quantitative Analysis of Small Volatile Molecules Using GC/MS with Various Ionizations techniques, e.g., EI, NICI, CI.
Operation
We are using two models of operation:
- Full Service: The Core Facility provides full service for the above analyses with the exception of LC method development, sample extraction, sample preparation, and data analysis that may be available through collaboration.
- Self Service: Work-in users are allowed. The training of work-in users is provided by the Core Facility Personnel. Training is required to ensure protection of instruments and optimization of results. The training is scheduled on an individual basis.
Fees
| Service Fees for Mass Spec Facility | Rate Per Sample/Service: NDUS Users |
Rate Per Sample/Service: Non-NDUS Users |
|---|---|---|
|
Small molecules 1. Simple sample analysis (up to 4 analytes)
|
$12
|
$30 |
|
2. Complex sample analysis (5 or more analytes)
|
$16 |
$45 |
|
3. Method development, simple sample analysis (up to 4 analytes) with UND maintaining IP rights
|
|
|
|
4. Method development, complex sample analysis (5 or more analytes) with UND maintaining IP rights
|
$900 |
$1,500 |
|
Protein analysis 1. Simple sample (up to 5 proteins)
|
|
|
|
2. Complex sample (5 or more proteins)
|
$45 |
$100 |
|
3. Method development with UND maintaining IP rights
|
$600 |
$1,500 |
Volume Discounts
Volume discounts are available on a case-by-case basis.
Financial Support
To apply for support funds, faculty may download the fee support application and send the completed form via e-mail to Dr. Mikhail Golovko at Mikhail.Golovko@UND.edu