Publications

2014-2015

Moritz AE, Rastedt DE, Stanislowski DJ, Shetty M, Smith M, Vaughan RA and Foster JD (2015) Reciprocal phosphorylation and palmitoylation control dopamine transporter kinetics. In press, J Biol Chem. PubMed
Kumar V, Yarravarapu N, Lapinsky DJ, Perley D, Felts B, Tomlinson MJ, Vaughan RA, Henry LK, Lever JR, Newman AH (2015) Novel Azido-Iodo Photoaffinity Ligands for the Serotonin Transporter Based on the Selective Serotonin Reuptake Inhibitor (S)-Citalopram. J Med Chem, 58:5609-5619. NCBI
Dahal R, Akula Bala P, Sharma B, Krout D, Foster JD, Cha J-H, Cao J, Newman AH, Lever JR, Vaughan RA and Henry LK (2014) Computational and biochemical docking of the irreversible cocaine analog RTI 82 directly demonstrates ligand positioning in the dopamine transporter central substrate-binding site. J Biol Chem. 289:29712-29727. Cover article. Highlighted in ASBMB Today, 11/2014, p.15. PubMed
Gaffaney JD, Shelty M, Akula-Bala P, Henry LK, Foster JD, and Vaughan RA (2014) Antagonist-induced conformational changes in dopamine transporter extracellular loop 2 involve residues in a potential salt bridge. Neurochemistry International, 73,16-26. PubMed

2012-2013

Moritz AE, Foster JD, Gorentla, BK, Mazei-Robison MS, Yang JW, Sitte HH, Blakely RD, and Vaughan RA (2013) Phosphorylation of dopamine transporter serine 7 modulates cocaine analog binding. J Biol Chem. 288:20-32. PubMed
Foster JD, Yang J-W, Moritz AE, Challa S, Smith MA, Holy M, Wilebski K, Sitte HH, and Vaughan RA (2012) Dopamine transporter phosphorylation site threonine 53 regulates substrate reuptake and amphetamine-stimulated efflux. J Biol Chem. 287:29702-29712. PubMed
Lapinsky DL, Yarravarapu N, Nolan TL, Surratt CK, Lever JR, Tomlinson M, Vaughan RA, and Deutsch HM (2012) Evolution of a Compact Photoprobe for the Dopamine Transporter Based on (±)- threo-Methylphenidate (Ritalin, Concerta). ACS Med Chem Lett 3:378-382.13. NCBI
Lapinksy DJ, Aggarwal S, Nolan TL, Surratt CK, Lever JR, Acharya R, Vaughan RA, Pandhare A, and Blanton MP (2012) (+)-2-(N-tert-Butylamino)-3’-[ 125I]-iodo-4’-azidopropiophenone: a Dopamine Transporter and Nicotinic Acetylcholine Receptor Photoaffinity Ligand Based on Bupropion (Wellbutrin, Zyban) Biorgan Med Chem Lett 22:523-526. NCBI

2011

Speed N, Saunders C, Davis AR, Owens WA, Matthies HJ, Saadat S, Kennedy JP, Vaughan RA, Neve RL, Lindsley CW, Russo SJ, Daws LC, Niswender1 KD, Galli A (2011) Impaired Striatal Akt Signaling Disrupts Dopamine Homeostasis and Increases Feeding. PLoS One. 6:e25169. PubMed
Foster JD and Vaughan RA (2011) Palmitoylation controls dopamine transporter kinetics, degradation, and protein kinase C dependent regulation. J Biol Chem 286:5175-5186. PubMed
Cremona ML, Bowton, E, Matties HJG, Speed N, Lute BJ, Sen M, Tobertson SD, Vaughan RA, Rothman JE, Galli A, Javitch JA, and Yamamoto A (2011) Flotillin-1 is essential for dopamine transporter endocytosis and reverse-transport of dopamine. Nat Neurosci 14:469-477. PubMed
Lapinsky DJ, Velagaleti R, Yarravarapu N, Liu Y, Huang Y, Surratt CK, Lever JR, Acharya R, Foster JD, and Vaughan RA (2011) Azido-iodo-N-benzyl derivatives of methylphenidate (Ritalin, Concerta): Rational design, synthesis, pharmacological evaluation, and dopamine transporter photoaffinity labeling. Biorgan Med Chem 19:504-512. PubMed

2009-2010

Cervinski MA, Foster JD, and Vaughan RA (2010) Syntaxin 1A regulates dopamine transporter activity, phosphorylation, and surface expression. Neurosci 170:408-416. PubMed
Speed N, Saadat S, Matthies H, Kennedy J, Vaughan RA, Javitch JA, Lindsley C, Russo S, Niswender K, and Galli A (2010) Akt-Dependent and Isoform-Specific Regulation of Dopamine Transporter Cell Surface Expression. ACS Chemical Neuroscience, 476-481.48:1067-1076. PubMed
Lapinsky DJ, Aggarwal S, Huang Y, Surratt CK, Lever JR, Foster JD, and Vaughan RA (2009) A novel photoaffinity ligand for the dopamine transporter based on pyrovalerone. Biorganic and Med Chem 17:3770-3774. PubMed
Gorentla BK, Moritz A, Foster JD, and Vaughan RA (2009) Proline-directed phosphorylation of the dopamine transporter N-terminal domain. Biochemistry. PubMed

2007-2008

Foster JD, Adkins S, Lever, JR, and Vaughan RA (2008) Phorbol ester-induced trafficking independent regulation and enhanced phosphorylation of the dopamine transporter associated with membrane rafts and cholesterol. J Neurochem 105:1683-1699. PubMed
Parnas ML, Zou MF, Duval, R, Lever JR, Newman AH, and Vaughan RA (2008) Labeling of dopamine transporter transmembrane domain 1 with the tropane ligand N-[4-(4-azido-3- [ 125I]iodophenyl)butyl]-2β-carbomethoxy-3β-(4-chlorophenyl)tropane implicates proximity of cocaine and substrate active sites. Mol Pharmacol 73:1141-1150. ASPET
Vaughan RA, Sakrikar DJ, Parnas, ML, Adkins, S, Foster, JD, Zou MF, Duval, R., Lever JR, Kulkarni, SS, and Newman AH (2007) Incorporation of the cocaine analog [125I]RTI 82 in transmembrane domain six of the dopamine transporter. J Biol Chem 282:8915-8925.

2005-2006

Newman, AH, Cha JH, Cao J, Kopajtic T, Katz JL, Parnas ML, Vaughan RA and Lever JR (2006) Design and Synthesis of a Novel and High Affinity Photoaffinity Ligand for the Dopamine and Serotonin Transporters Based on 2β-Carbomethoxy-3β-Biphenyltropane. J Med Chem 49:6621-6625 12. ACS
Henry LK, Field JR, Adkins EM, Parnas ML, Vaughan RA, Newman AH, and Blakely RD (2006) Antidepressant Recognition by Serotonin Transporters: TMs I And III Interact To Establish High Affinity Citalopram Recognition. J Biol Chem 281:2012-2023.
Cervinski MA, Foster, JD and Vaughan RA (2005) Psychostimulant substrates stimulate phosphorylation and down regulation of dopamine transporters by transport and protein kinase C dependent mechanisms. J Biol Chem 280:40442-40449. PubMed
Gorentla BK and Vaughan RA (2005) Differential effects of dopamine and psychostimulant drugs on dopamine transporter phosphorylation and regulation. Neuropharmacology 49:759-768. (Special issue on neurotransmitter transporters.) PubMed
Lever J, Zou MF, Parnas ML, Duval RA, Wirtz SE, Justice JB, Vaughan RA, and Newman AH (2005) Radioiodinated azide and isothiocyanate derivatives of cocaine for irreversible labeling of dopamine transporters: synthesis and covalent binding studies of [125I]-MFZ-2-24 AND [125I]-MFZ-3-37. Bioconjug Chem 16:644-649. PubMed

2001-2004

Gaffaney JD and Vaughan RA (2004) Uptake inhibitors but not substrates induce protease resistance in extracellular loop two of the rat dopamine transporter. Mol Pharmacol 65:692-701. PubMed
Chagkutip J, Vaughan RA, Govitrapong P, Ebadi M (2003) 1-Methyl-4-phenylpyridinium-induced down-regulation of dopamine transporter function correlates with a reduction in dopamine transporter cell surface expression. Biochem Biophys Res Commun. 311:49-54. PubMed
Foster J, Pananusorn B, Cervinksi M, Holden HE and Vaughan RA (2003) Dopamine transporters are dephosphorylated in striatal homogenates and in vitro by protein phosphatase 1. Mol Brain Res 110:100-108. PubMed
Foster J, Pananusorn B and Vaughan RA (2002) Dopamine transporters are phosphorylated on Nterminal serines in rat striatum. J Biol Chem 277:25178-25186. PubMed
Chen N, Vaughan RA, and Reith MEA (2001) The role of conserved tryptophan and acidic residues in the human dopamine transporter as characterized by site-directed mutagenesis. J Neurochem 77:1116- 1127. PubMed
Vaughan RA, Gaffaney JD, Wang N, Lever J, Reith MEA, and Dutta A (2001) Dual incorporation sites for photoaffinity ligands on the dopamine transporter implicate proximity of labeled domains. Mol Pharmacol 59:1157-1164. PubMed
Dutta A, Fei X-S, Vaughan RA, Gaffaney JD, Wang N, Lever J, and Reith MEA (2001) Design, synthesis, and characterization of 4-[2-(diphenylmethoxy)ethyl]-1-benzyl piperidine, a novel dopamine transporter photoaffinity label. Life Sci 68:1839-1849. PubMed

1999-2000

Thompson AC, Zapata A, Vaughan RA, Justice JB, Sharpe LG, and Shippenberg T (2000) Kappa-opioid receptor activation regulates dopamine uptake and opposes the effects of cocaine. J Neurosci 20:9333-9340. PubMed
Bauman AL, Apparsundaram S, Ramamoorthy S, Wadzinski BE, Vaughan RA, and Blakely RD (2000) Cocaine and antidepressant-sensitive biogenic amine transporters exist in regulated complexes with protein phosphatase 2A. J Neurosci 20:7571-7578.11. PubMed
Letchworth SR, Sexton T, Vaughan RA, Vrana K, Childers S, and Porrino L (1999) Chronic cocaine administration differentially regulates dopamine transporter mRNA and protein. J Neurochem 73:1982-1989.
Garzon M, Vaughan RA, Uhl GR, Kuhar MJ, and Pickel VM (1999) Cholinergic axon terminals in the ventral tegmental area target a subpopulation of neurons expressing low levels of the dopamine transporter. J Comp Neurol 410:197-210. PubMed
Vaughan RA, Agoston GE, Lever J, and Newman AH (1999) Differential binding of tropane-based photoaffinity ligands on the dopamine transporter. J Neurosci 19:630-636. PubMed

1997-1998

Kokoshka JM, Vaughan RA, Hanson GR and Fleckenstein AE (1998) Nature of methamphetamine-induced rapid and reversible changes in dopamine transporters. Eur J Pharmacol 361:269-275. PubMed
Boja JW, Carroll FI, Vaughan RA, Kopajtic T, and Kuhar MJ (1998) Multiple binding sites for [ 125I]RTI-121 and other cocaine analogs in rat frontal cerebral cortex. Synapse 30:9-17. PubMed
Agoston GE, Vaughan RA, Lever J, Izenwasser S, Terry PD, and Newman AH (1997) A novel photoaffinity label for the dopamine transporter based on N-substituted-4’4’-difluoro-3a- (diphenylmethoxy)tropane. Bioorganic and Medicinal Chemistry Lett. 7:3027-3032. Miami University
Vaughan RA, Huff RA, Uhl GR, and Kuhar MJ (1997) Protein kinase C-mediated phosphorylation and functional regulation of dopamine transporters in rat striatum. J Biol Chem 272:15541-15546. PubMed
Nirenberg MJ, Vaughan RA, Uhl GR, Kuhar MJ, and Pickel VM (1997) The dopamine transporter: comparative ultrastructure of dopaminergic axons in the limbic and motor compartments of the nucleus accumbens. J Neurosci 17: 6899-6907. PubMed
Nirenberg MJ, Vaughan RA, Uhl GR, Kuhar MJ, and Pickel VM (1997) Immunogold localization of the dopamine transporter: An ultrastructural study of the rat ventral tegmental area. J Neurosci 17:4037-4044. PubMed
Huff RA, Vaughan RA, Kuhar M, and Uhl G (1997) Dopamine transporters: phorbol esters enhance phosphorylation and decrease Vmax. J Neurochem 68:225-232.

1995-1996

Vaughan RA and Kuhar MJ (1996) Dopamine transporter ligand binding domains: structural and functional properties revealed by limited proteolysis. J Biol Chem 271:21672-21680. PubMed
Vaughan RA, Brown VL, McCoy MT. and Kuhar MJ (1996) Species- and brain-region specific dopamine transporters: immunological and glycosylation characteristics. J Neurochem 66:2146-2152. Online Library
Nirenberg MJ, Vaughan RA, Uhl GR, Kuhar MJ, and Pickel VM (1996) The dopamine transporter is localized to dendritic and axonal plasma membranes of nigrostriatal dopaminergic neurons. J Neurosci 16:436-447. PubMed
Revay R, Vaughan R, and Kuhar MJ (1996) Dopamine transporter immunohistochemistry in median eminence, amygdala and other areas. Synapse 22:93-99.10. PubMed
Freed CR, Revay R, Vaughan RA, Kreik E, Uhl G, and Kuhar MJ (1995) Dopamine transporter immunoreactivity in rat brain. J Comp Neurol 359:340-349. PubMed
Vaughan RA (1995) Photoaffinity-labeled ligand binding domains on dopamine transporters identified by peptide mapping. Mol Pharmacol 47:956-964. PubMed

1990-1994

Hereld D, Vaughan RA, Kim JY, Borleis J, and Devreotes P (1994) Localization of ligand-induced phosphorylation sites to serine clusters in the C-terminal domain of the Dictyostelium cAMP receptor, cAR 1. J Biol Chem 269:7036-7044. JBC
Patel A, Cerruti C, Vaughan RA, and Kuhar MJ (1994) Developmentally regulated glycosylation of dopamine transporters. Dev Brain Res 83:53-58. ScienceDirect
Vaughan R, Uhl G, and Kuhar M. (1993) Recognition of dopamine transporters by anti-peptide antibodies. Mol Cell Neurosci 4:209-215. ScienceDirect
Lew R, Patel A, Vaughan RA, Wilson A, and Kuhar MJ (1992) Microheterogeneity of dopamine transporters in rat striatum and nucleus accumbens. Brain Research 584:266-271. ScienceDirect
Lew R, Vaughan R, Simantov R, Wilson A, and Kuhar MJ (1991) Dopamine transporters in nucleus accumbens and the striatum have different apparent molecular weights. Synapse 8:152-153. Wiley Online
Vaughan RA, Simantov R, Lew R, and Kuhar MJ (1991) A rapid binding assay for solubilized dopamine transporters using [3H]WIN 35,428. J Neurosci Meth 40:9-16. PubMed
Simantov R. Vaughan R, Lew R, Wilson A, and Kuhar MJ (1991) Dopamine transporter-cocaine receptor: characterization and purification. Adv Biosci 82:151-154. ScienceDirect
Johnson RL, Vaughan RA, Caterina MJ, Van Haastert PJM, and Devreotes PN (1991) Overexpression of the cAMP receptor 1 (cARl) in growing Dictyostelium cells. Biochemistry 30:6982- 6986. PubMed

1987-1988

Vaughan RA and Devreotes PN (1988) Ligand-induced phosphorylation of the cAMP receptor from Dictyostelium discoideum. J Biol Chem 263:14538-14543. PubMed
Vaughan RA and Rutherford CL (1987) Distribution of cyclic AMP dependent protein kinase during development of Dictyostelium discoideum. Dev Biol 121:359-367. PubMed
Klein P, Vaughan R, Borleis J, and Devreotes P (1987) The surface cAMP receptor in Dictyostelium: levels of ligand-induced phosphorylation, solubilization, identification of primary transcript, and developmental regulation of expression. J Biol Chem 262:358-364. PubMed

Invited Articles and Book Chapters

Foster JD, Henry LK, and Vaughan RA and (2016) RTI 82 and ligand protein interactions. In “The Neuroscience of Cocaine: Mechanisms and Treatments” Academic Press, Ed. Victor R. Preedy. In preparation.
Foster JD, Rastedt D, Challa Siva Kanaka S, and Vaughan RA (2014) Analysis of Neurotransmitter Transporter Posttranslational Modifications: Phosphorylation and Palmitoylation. In press, Neuromethods Ed. H. Bonisch and H. Sitte.
Vaughan RA and Foster JD (2013) Mechanisms of dopamine transporter regulation in normal and disease states. Trends Pharmacol Sci 34:489-496.
Parnas ML and Vaughan RA (2008) Molecular Structure and Organization of Dopamine Transporters. In “Dopamine Transporters: Chemistry, Biology, and Pharmacology” Wiley Series in Drug Discovery and Development (ed. S. Izenwasser and M.Trudell). pp. 73-95. (Cover article).
Foster JD, Cervinski MA, Gorentla B, and Vaughan RA (2006) Regulation of dopamine transporter by phosphorylation. Handb Exp Pharmacol 175:197-214 Michael Freissmuth, ed.15
Parnas ML and Vaughan RA (2005) Dopamine transporter. XPharm database, Elsevier. D. Bylund, Ed.
Sakrikar DS and Vaughan RA (2005) Norepinephrine transporter. XPharm database, Elsevier. D.Bylund, Ed.
Vaughan RA, Parnas ML, Gaffaney JD, Lowe MJ, Wirtz S., Pham A, Reed B, Dutta SM, Murray KK, and Justice JB (2005) Affinity labeling the dopamine transporter ligand binding site. J Neurosci Methods 143:33-40.
Vaughan RA (2004) Phosphorylation and Regulation of Psychostimulant-Sensitive Neurotransmitter Transporters. Perspectives in Pharmacology. J Pharmacol Exp Therap 310:1-7. (Cover Article).
Lowe MJ, Gaffaney JD and Vaughan RA (2002) Substrate recognition and interactions at the dopamine transporter. Receptor Biochemistry and Methodology Wiley Publishers, (David Sibley, Ed.). pp.161-177.
Vaughan RA (2000) Regulation of the dopamine transporters by phosphorylation and impact on cocaine action. Cerebral Signal Transduction: From First to Fourth Messengers (M.E.A. Reith, Ed.), Humana Press, Totowa, N.J. pp. 375-400.
Vaughan RA, Huff RA, Uhl GR, and Kuhar MJ (1998) Phosphorylation of dopamine transporters and rapid adaptation to cocaine. In, “Catecholamines: Bridging basic science with clinical medicine.” Adv Pharmacol (D. Goldstein, Ed.), vol. 42, pp. 1042-1045.
Kuhar MJ, Vaughan RA, Uhl G, Cerruti C, Revay R, Freed C, Nirenberg M, and Pickel V. (1998) Localization of the dopamine transporter protein by microscopic histochemistry. In, “Catecholamines: Bridging basic science with clinical medicine.” Adv Pharmacol (D. Goldstein, Ed.), vol. 42, pp. 168-170.
Vaughan RA (1998) GBR and Cocaine Photoaffinity Ligands as Probes for Dopamine Transporter Structure. Methods in Enzymology, Neurotransmitter Transporters. (S. Amara, Ed.). vol. 296, pp. 219-230.
Boja JW, Vaughan RA, Patel A, Shaya E, and Kuhar MJ (1994) The Dopamine Transporter. Dopamine Receptors and Transporters. (H. Niznik, Ed.) Marcel Dekker, Inc., New York, pp. 611-644.
Pitt GS, Gundersen RE, Lilly PJ. Pupillo MB, Vaughan RA, and Devreotes PN (1990) G Protein-linked signal transduction in aggregating Dictyostelium. Soc Gen Physiol Ser 45:125-131.
Pupillo M, Klein P, Vaughan R, Pitt G, Lilly P, Sun J, Devreotes P, Kumaggai A, and Firtel R (1989) cAMP receptor and G-protein Interactions control development in Dictyostelium. Cold Spring Harbor Symp. on Quant.Biol. Vol Lll, 657-665.
Gundersen R, Johnson R, Sun T, Pupillo M, Lilly P, Vaughan R, and Devreotes P (1989) Reversible phosphorylation of G protein-coupled receptors controls cAMP oscillations in Dictyostelium. Theoretical Models for Cell to Cell Signaling. (A. Goldbeter, Ed.). Academic Press London, pp.477- 486. 14
Johnson R, Gundersen R, Lilly P, Pitt G, Pupillo M, Sun T, Vaughan R, and Devreotes P (1989) Gprotein linked signal transduction systems control development in Dictyostelium. British Soc. Dev. Biol., St. Andrews, Scotland, Development (Supplement) 107:75-80.
Vaughan RA, Johnson R, Caterina M, and Devreotes P (1989) Adaptation of chemoattractant elicited responses in Dictyostelium discoideum. NATO Conference on Sensing and Response, The Netherlands, Transducing Pathways: Activation and Desensitization. (T. Konijn, et al., Eds.).
Lilly P, Klein P, Theibert A, Vaughan R, Pupillo M, Saxe C, Kimmel A, and Devreotes PN (1988) Receptor-G protein interactions in the development of Dictyostelium. Botanica Acta 101:123-127.
Vaughan R, Pupillo M, Theibert A. Klein P, and Devreotes P (1986) Surface receptor mediated activation and adaptation of adenylate cyclase in Dictyostelium discoideum. NATO/ASI Workshop on Desensitization. Series H., Vol. 6. pp.15-22. (T. Konijn, ed.)
Rutherford CL, Vaughan RA, Cloutier MJ, Naranan V, Brickey DA, and Ferris DK (1985) Compartmentation in Dictyostelium. Ann Rev of Microbiol 39:271-289.

Manuscripts in Preparation

ChallaSivaKanaka S, Sitte HH, Foster JD, Vaughan RA (2015) Psychostimulant substrates stimulate phosphorylation of dopamine transporter proline directed site threonine 53.
Rastedt D, Vaughan RA, Foster JD (2015) Multiple palmitoyl acyltransferases modify DAT palmitoylation. In preparation.
Zhen J, Moritz AE, Foster JD, Reith MEA, Vaughan RA (2015) Phosphorylation of dopamine transporter serine 13 drives amphetamine-stimulated DA efflux via complex interactions with intracellular domains. In preparation.
Oh, R, Moritz AE, Foster JD, Sitte H, Vaughan RA, Shippenberg TS, Ramamoorthy S, (2015) Kappa opioid regulation of dopamine uptake via ERK-dependent phosphorylation of dopamine transporter Threonine 53. In preparation.
Pramod AB, Dahal RA, Sharma B, Krout D, Foster JD, Zou M-F, Boatang C, Amy Hauck Newman AH, Lever JR, Henry LK*, and Vaughan RA* (2015) Adduction of the Photoactive Azide of Irreversible Cocaine Analog, [125I] MFZ 2-24 Elucidates the Cocaine Binding Site in the Dopamine Transporter. *co-corresponding authors.
Singer BF, Guptaroy B, Austin CJ, Wohl I, Vaughan RA, Gnegy ME, Robinson TE, and Aragona BJ (2015) Individual Variation in Incentive Salience Attribution and Accumbens Dopamine Transporter Expression and Function. In review, J Neurosci