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Light Microscopy Core
Monday through Friday - 8 a.m.-4:30 p.m.
Users who have received training and are wanting to use the Light Microscopy Core after hours can obtain a key from Sarah Abrahamson. Policies regarding the signing out of keys and use of the facility after hours are listed below.
Suite W162 on the first floor of the new Medical Sciences Building; 1301 N Columbia Road.
Confocal Microscope Systems
Zeiss LSM-510 Meta Confocal Microscope
Equipped with an Argon laser (458, 488, 514nm excitation) and two Helium-Neon Lasers (543 nm and 633 nm excitation). The spectral META detector provides the ability to separate fluorochrome signals with overlapping emission characteristics and thus further increases flexibility for studies involving multiple fluorescent labels. The system is mounted on a Zeiss Axiovert 200M inverted microscope.
Note: New trainees will be taught using the ZEN software only. The AIM software will be available for previous users who have completed their training on the AIM software to utilize, though learning the new ZEN software is recommended.
- Available Dichroics and Filters
- Setting up a New Configuration
- Configuration Practice Sheet 1
- Configuration Practice Sheet 2
- Camera Alignment Macro
- Available Objectives
- ZEN Software
Olympus Fluoview 300 Confocal Microscope
Equipped with an Argon laser (488 nm excitation) and a Helium-Neon laser (543 nm excitation). This system supports confocal imaging of green and red emitting fluorophores. The system is mounted on an Olympus IX70 inverted microscope.
Olympus FV1000 MPE Basic Multiphoton Microscope
The system is mounted on an Olympus BX61WI upright microscope and equipped with a mode locked Ti:sapphire laser (690-1040nm), a multi Argon laser (457nm, 488nm, 515nm, Total 30mW), a HeNe laser (543nm, 1mW), and a 635nm HeNe laser. The system is configured for a wide range of applications including intravital microscopy using small animals.
Nikon Eclipse TE300 (inverted) Fluorescent Microscope
Equipped with a Hamamatsu digital camera. A MAC5000 excitation filter wheel allows FURA2 imaging at 340/380 nm. Emission filters are available for FITC, Rhodamine, EGFP, BFP, and EYFP.
Nikon Eclipse 80i (upright) Fluorescent Microscope
Equipped with a SPOT II digital camera capable of capturing black and white as well as color images. Emission filters are available for DAPI, FITC, Rhodamine, and Texas Red.
Olympus TIRF Microscope
Equipped with a 445, 491, 514, and 561 lasers. It can perform both widefield fluorescence and TIRF applications. Two different cameras are available; a Hammamatsu RCA flash 4.0 camera or an Andor iXon Ultra camera. The system is mounted on an inverted Olympus IX83 microscope.
This instrument was purchased with funds from a NIH Shared Instrumentation Grant; Grant #1S100D016250-01. Please acknowledge this grant when reporting studies using this system.
- Available Filtersets
- Available Objectives
- On-Off Procedure
- Single Wavelength Acquisition
- Single Wavelength TIRF Acquisition
- Attofluor Metal Chambers (3) that hold 25mm round coverslips
- Temp Control 37-2 Digital Dish Warmer (for use on the Zeiss only)
- Harvard Apparatus Temperature controller; model TC-202A
- Medical Systems Corp: Open perfusion Micro-incubator; model PDMI-2
- Workstation Computer: Includes Imaris, Photoshop, Image J, Zeiss ZEN software, and Olympus FV10 ASW Viewer
- Sanyo CO2 Incubator; model MCO-5AC
- Small refrigerator